There are few studies on the effects of copper chlorophylline (Cu-chl) and magnesium chlorophylline (Mg-chl) on the cellular level, physiological parameters and DNA damage to Culex pipiens larvae. These parameters may be conducive to larval death as a result of photosensitisation reaction after feeding on these photosensitisers and being exposed to light. This study focuses on the impact of Cu-chl and Mg-chl on ultrastructural damage and cellular alteration, immune parameters and DNA damage. The results showed a significant change in the phenoloxidase activity as a function of photosensitiser concentrations. The ultrastructure of midgut tissues revealed obvious damages in different cellular levels. There was no significant effect on DNA. So, it can be concluded that Cu-chl and Mg-chl caused C. pipiens larvae death via the physical damage of larval tissues, and the direct effect on biochemical and physiological parameters of larval viability. Healthy DNA molecules, after the larval treatment, reflected no chance for the undesired mutation of the sublethal concentration of Cu-chl and Mg-chl.