Two new species of Gentianella are described. Gentianella galtonioides J.S. Pringle, from Peru, differs from G. kusnezowii in its leaves with 11 to 17 primary veins and non-sheathing bases, and in its white corollas with elliptic-rhombic lobes. Gentianella pharos J.S. Pringle, from Bolivia, differs from G. florida in its more elongate inflorescences and distinctly yellow corollas, from G. pluvialis in its smaller flowers and less deeply lobed corollas, and from both in its smaller size and deltoid calyx lobes. The distinctness of Gentiana longipes and G. totorensis is discussed, and the species are transferred to Gentianella. Gynodioecy is confirmed in Gentianella pluvialis with the report of specimens with bisexual flowers.