Understanding the patterns of population connectivity and level of genetic diversity can facilitate the identification of both ecologically relevant populations and the spatial scales at which conservation management may need to focus. We quantified genetic variation within and among populations of black-throated finches across their current distribution. To quantify genetic structure and diversity, we genotyped 242 individuals from four populations using 14 polymorphic microsatellite markers and sequenced 25 individuals based on a 302-base-pair segment of mitochondrial control region. We found modest levels of genetic diversity (average allelic richness r = 4.37 ± 0.41 (standard error) and average heterozygosity H_O = 0.42 ± 0.040 (standard error)) with no bottleneck signature among sampled populations. We identified two genetic groups that represent populations of two subspecies based on Bayesian clustering analysis and low levels of genetic differentiation based on pairwise genetic differentiation statistics (all F_ST, R_ST and Nei’s unbiased D values < 0.1). Our data suggest that genetic exchange occurs among sampled populations despite recent population declines. Conservation efforts that focus on maintaining habitat connectivity and increasing habitat quality to ensure a high level of gene flow on a larger scale will improve the species’ ability to persist in changing landscapes. Conservation management should also support continuous monitoring of the bird to identify any rapid population declines as land-use intensification occurs throughout the species’ range.

Australian native fauna are thought to be well adapted to fire-prone landscapes, but bushfires may still pose considerable challenges or stressors to wildlife. We investigated the impact of bushfire on the stress physiology of the woylie (brush-tailed bettong, Bettongia penicillata) a critically endangered Australian marsupial, and assessed whether fitness indices (body condition and parasite load) influenced stress physiology before and after the fire. We hypothesised that there would be a significant change in stress physiology indicators (in the form of faecal cortisol metabolites, FCM) following the fire, compared with the months previous. We trapped woylies (n = 19) at Whiteman Park Reserve in Perth, Western Australia, two days after a major bushfire and measured FCM concentration by enzyme immunoassay. Population-level comparisons of FCM were made between these samples and those collected in previous months (n = 58). While mean FCM varied by month of sample collection, it was not higher after the fire. We suggest that woylies may be able to maintain homeostasis through change (allostasis), at least in the period immediately after the fire. This is supported by our finding that FCM did not relate significantly to body condition or parasite load. Our results potentially highlight the physiological and behavioural adaptations of woylies to fire, which could be further explored in future studies.

Litoria nannotis is an endangered waterfall frog from the wet tropics region in north Queensland that has suffered significant population declines due to the emerging fungal disease known as chytridiomycosis. The species has two deeply divergent lineages, and we used 454 shotgun sequencing of DNA extracted from one individual of the northern lineage to identify and design PCR primers for 576 microsatellite loci. Thirty markers were tested for amplification success and variability in a population sample from each lineage. Of these, 17 were found to be polymorphic in the northern lineage and 10 loci were polymorphic in the southern lineage. Numbers of alleles per locus ranged from 2 to 14 (mean = 6.47, s.d. = 4.02) for the northern lineage (17 polymorphic loci), and from 2 to 8 (mean = 5.40, s.d. = 2.55) in the southern lineage (10 polymorphic loci). Levels of heterozygosity were high in both lineages (northern mean H_E = 0.63, s.d. = 0.21, range = 0.27–0.89; southern mean H_E = 0.57, s.d. = 0.25, range = 0.18–0.81). These loci will be useful in understanding the genetic variation and connectivity amongst populations of this species recovering from mass population declines due to disease.

Whilst flehmen behaviour has been observed in some marsupials, including the southern hairy-nosed wombat (Lasiorhinus latifrons), it is unknown whether it, or other male-specific behaviours are exhibited differentially in response to the detection of oestrous females, which if proven, could assist in the detection of oestrus in breeding programs for captive southern hairy-nosed wombats. Six captive male southern hairy-nosed wombats were exposed to urine samples collected from a single female that was confirmed to be in oestrus and mid-pregnancy. Urine samples were presented to the males simultaneously and subsequent behaviours recorded for five minutes. There were no significant differences in the number of times flehmen behaviour was displayed in response to oestrous and non-oestrous urine samples (P = 0.577), the number of times the male sniffed each sample (P = 0.595), or the time spent investigating each urine sample (P = 0.414). Our results suggest that neither male flehmen behaviour nor any specific male-related precopulatory behaviour was associated with the olfaction of urine from a female in different stages of the oestrous cycle, and is thus unlikely to be a reliable management tool for the detection of oestrus.

Habitat loss and fragmentation are key threats to local koala (Phascolarctos cinereus) populations. Broad-scale management is suboptimal for koalas because distribution models are not easily generalised across regions. Therefore, it is imperative that data relevant to local management bodies are available. Genetic data provides important information on gene flow and potential habitat barriers, including anthropogenic disturbances. Little genetic data are available for nationally significant koala populations in north-eastern New South Wales, despite reported declines due to urbanisation and habitat loss. In this study, we develop 14 novel microsatellite loci to investigate koala populations in north-eastern New South Wales (Port Macquarie, Coffs Harbour, Tyagarah, Ballina) and south-eastern Queensland (Coomera). All locations were significantly differentiated (F_ST = 0.096–0.213; F′ _ST = 0.282–0.582), and this pattern was not consistent with isolation by distance (R² = 0.228, P = 0.058). Population assignment clustered the more northern populations (Ballina, Tyagarah and Coomera), suggesting contemporary gene flow among these sites. For all locations, low molecular variation among (16%) rather than within (84%) sites suggests historical connectivity. These results suggest that koala populations in north-eastern New South Wales and south-eastern Queensland are experiencing contemporary impediments to gene flow, and highlight the importance of maintaining habitat connectivity across this region.

Population monitoring is fundamental to the conservation of threatened species. This study aimed to develop an effective approach for long-term monitoring of the yellow-bellied glider (Petaurus australis) in north-east New South Wales. We conducted repeat surveys to account for imperfect detection and used counts in abundance modelling to produce indices of abundance. We used simulations to explore refinements to our study design. Surveys over three consecutive years produced 195 detections with >95% of detections by call. The probability of detection varied across years and survey occasions, ranging from 0.22 to 0.71. Abundance estimates were remarkably constant across years, ranging from 2.3 ± 0.5 to 2.4 ± 0.6 individuals per site. Occupancy estimates were also constant across years (0.90–0.91). Simulations were run to investigate the influence of the number of surveys (2 or 3) and the number of survey sites (20, 40 or 50) on the probability of occupancy. The design that reduced bias and provided an adequate improvement to precision was that of three visits to 40 survey sites. This design should be adequate to detect a decline in population abundance. Further studies of this kind are needed to better understand the population dynamics of this species.

Body condition is an important aspect of the health of any animal. The current standard method of body condition assessment in the platypus is the tail volume index (TVI). Although the tail is the largest repository of fat in the platypus, the reliability of TVI has not been adequately demonstrated. The aims of this study were, first to assess performance of the TVI, and second, to develop and evaluate performance of new techniques for routine field assessment of platypus body condition. Morphometric data were collected under anaesthesia from 137 adult wild platypuses (74 males, 63 females) captured in north-west Tasmania; ultrasound images of tail fat were also collected from 100 of these individuals (54 males, 46 females). Three new indices for platypus body condition were identified. An objective tail fat index (Relative Tail Fat Volume: RTFV_TBL) was developed, based on cross-sectional area measurements taken from detailed ultrasound images compared with total body length (TBL). Two body condition indices intended for routine field use were developed – one based on body mass (m_b) and bill width (BW) (Body Condition Index; BCI_BW), and the other based on a single linear ultrasonographic measurement of tail fat depth and BW (Relative Fat Depth; RFD_BW). Results indicated that RFD_BW outperforms TVI as an index of platypus tail fat. Further work, however, is needed to determine the relationship between tail fat and total body fat in the platypus before conclusions can be drawn about the effectiveness of BCI_BW as a body condition index.

To study the genetic diversity of the Eurasian coot (Fulica atra), 16 novel microsatellites were isolated and characterised using a (AC)₁₂-enriched genomic library. Polymorphism of each locus was detected in 19 individuals from Weishan Lake and Zhaoyang Lake, both located in Shandong Province, China. These microsatellite loci revealed 3–12 alleles in two populations. One locus (Fa6) showed significant deviations from Hardy–Weinberg equilibrium in the Zhaoyang Lake population. By means of Principal Coordinates Analysis, 19 individuals were divided into two groups, which verified they were from two populations. These novel microsatellites will be useful for further studies and genetic analyses of the Eurasian coot.

The distribution and taxonomy of varanid lizards occurring in the Bismarck Archipelago is revised on the basis of field surveys, examination of museum vouchers and molecular phylogenetic analyses. A total of four species is recorded: Varanus indicus and Varanus finschi on New Britain and some of its offshore islands, Varanus douarrha on New Ireland, Lavongai and Djaul, and Varanus semotus on Mussau Island. V. douarrha, previously mistaken for both V. indicus and V. finschi, is shown to be the only species represented on New Ireland and is here resurrected as a valid taxon based on an integrated approach combining morphological and molecular evidence. Phylogenetic analyses of two mitochondrial genes suggest that V. indicus is a relatively recent immigrant to the Bismarck Islands, whereas V. douarrha, V. finschi and V. semotus have significantly longer histories in the island group. Together with the recently described V. semotus the revalidation of V. douarrha doubles the number of species known to occur in the Bismarck region and highlights an important component of both local and regional endemism.