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12 May 2011 Lactate Dehydrogenase C and Energy Metabolism in Mouse Sperm
Fanny Odet, Scott A. Gabel, Jason Williams, Robert E. London, Erwin Goldberg, Edward M. Eddy
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We demonstrated previously that disruption of the germ cell-specific lactate dehydrogenase C gene (Ldhc) led to male infertility due to defects in sperm function, including a rapid decline in sperm ATP levels, a decrease in progressive motility, and a failure to develop hyperactivated motility. We hypothesized that lack of LDHC disrupts glycolysis by feedback inhibition, either by causing a defect in renewal of the NAD cofactor essential for activity of glyceraldehyde 3-phosphate dehydrogenase, sperm (GAPDHS), or an accumulation of pyruvate. To test these hypotheses, nuclear magnetic resonance analysis was used to follow the utilization of labeled substrates in real time. We found that in sperm lacking LDHC, glucose consumption was disrupted, but the NAD:NADH ratio and pyruvate levels were unchanged, and pyruvate was rapidly metabolized to lactate. Moreover, the metabolic disorder induced by treatment with the lactate dehydrogenase (LDH) inhibitor sodium oxamate was different from that caused by lack of LDHC. This supported our earlier conclusion that LDHA, an LDH isozyme present in the principal piece of the flagellum, is responsible for the residual LDH activity in sperm lacking LDHC, but suggested that LDHC has an additional role in the maintenance of energy metabolism in sperm. By coimmunoprecipitation coupled with mass spectrometry, we identified 27 proteins associated with LDHC. A majority of these proteins are implicated in ATP synthesis, utilization, transport, and/or sequestration. This led us to hypothesize that in addition to its role in glycolysis, LDHC is part of a complex involved in ATP homeostasis that is disrupted in sperm lacking LDHC.

Lack of LDHC impairs mouse sperm glycolysis but not pyruvate to lactate conversion.

Fanny Odet, Scott A. Gabel, Jason Williams, Robert E. London, Erwin Goldberg, and Edward M. Eddy "Lactate Dehydrogenase C and Energy Metabolism in Mouse Sperm," Biology of Reproduction 85(3), 556-564, (12 May 2011).
Received: 3 March 2011; Accepted: 1 April 2011; Published: 12 May 2011

gamete biology
nuclear magnetic resonance
null mutation/knockout
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