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9 November 2017 Nonrandom contribution of left and right testes to germline transmission from mouse spermatogonial stem cells
Mito Kanatsu-Shinohara, Honda Naoki, Takashi Shinohara
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Abstract

Vast amounts of sperm are produced from spermatogonial stem cells (SSCs), which continuously undergo self-renewal. We examined the possible effect of laterality in male germline transmission efficiency of SSCs using a spermatogonial transplantation technique. We transplanted the same number of wild-type and Egfp transgenic SSCs in the same or different testes of individual recipient mice and compared the fertility of each type of recipient by natural mating. Transgenic mice were born within 3 months after transplantation regardless of the transplantation pattern. However, transgenic offspring were born at a significantly increased frequency when wild-type and transgenic SSCs were transplanted separately. In addition, this type of recipient sired significantly more litters that consisted exclusively of transgenicmice, which suggested that left and right testes have different time windows for fertilization. Thus, laterality plays an important role in germline transmission patterns from SSCs.

Summary Sentence

Nonrandomness of germline transmission pattern of spermatogonial stem cells was examined by spermatogonial transplantation.

© The Author(s) 2017. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Mito Kanatsu-Shinohara, Honda Naoki, and Takashi Shinohara "Nonrandom contribution of left and right testes to germline transmission from mouse spermatogonial stem cells," Biology of Reproduction 97(6), 902-910, (9 November 2017). https://doi.org/10.1093/biolre/iox141
Received: 26 June 2017; Accepted: 7 November 2017; Published: 9 November 2017
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KEYWORDS
spermatogenesis
spermatogonial stem cells
testis
Transplantation
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