In this study, the influence of feeding system on the fatty acid (FA) profile of suckler beef (SB), moderate grain-fed (MG), and grass-fed (GF) beef was compared. It was found that total saturated FA content was not affected; however, greater 14:0, total polyunsaturated FA content, and lower n-6/n-3 ratio suggest some transmittance of milk FA in SB calves (P < 0.001).

A total of sixteen barrows were randomly assigned to diets containing 5% biohydrogenation product (BHP)-enriched or control beef fat for 7 weeks. On completion of 7 weeks, we found that feeding enriched fat led to deposition of BHP and isomer-specific metabolism of trans-18:1 in adipose tissue. It was also noticed that total and HDL-cholesterol were decreased; however, LDL-cholesterol and triglycerides were not affected.

Five essential oil compounds in four doses (0, 50, 500, and 2000 mg kg^-1 of fresh forage) were sprayed on ryegrass forage before ensiling. Thymol, eugenol, and cinnamaldehyde at 2000 mg and carvacrol at 500 and 2000 mg inhibited deamination. Cinnamaldehyde at 2000 mg resulted in silages with 9.8% higher true protein.

Aging explained >45% of the variability in beef tenderness, whereas electrical stimulation explained >12%. The effect of electrical stimulation was significant for calf-fed steers up to 27 d of aging. However, this effect did not persist beyond 6 d of aging for yearling-fed steers. However, electrical stimulation prevents cold toughening in lighter, leaner carcasses.

The aim of the study was to investigate the effects of melatonin implants on cashmere growth and productive performance of cashmere goats. A total of thirty female goats were assigned to one of three treatments (n = 10), including control and two treatments where melatonin [2 mg kg^-1 body weight (BW)] was implanted either in April and June or in June. Compared with the control, implantation in April and June increased cashmere yield and maximum cashmere length by 20.3% and 15.7%, respectively (P < 0.01), with an average initiation date of 22 May 2013 and cessation date of 26 Mar. 2014. In contrast, no cashmere growth was observed in control goats until 19 June 2013 and the growth ceased on 3 Apr. 2014. Melatonin only implanted in June had no effect on cashmere yield and maximum cashmere length, with an average initiation date of 5 June 2013 and cessation date of 27 Mar. 2014. Cashmere growth rate, cashmere fiber diameter, the final BW, and average daily gain were not influenced by melatonin implantation. Results suggested that melatonin implantation during the cashmere nongrowing period is an effective way to stimulate cashmere growth and extend the cashmere growth phase with April and June identified as the most appropriate time for implantation.

The aim of the study was to determine the effect of dietary vitamin E level on cholesterol and vitamin E content and fatty acid profile in broiler chicken breast and leg muscles. Chickens (420 cocks) were randomly divided into two feeding groups (210 birds each). The control group was fed with standard diet containing 44 mg kg^-1 of vitamin E, whereas the experimental group was supplemented with extra 200 mg kg^-1 of vitamin E. The dietary addition of vitamin E caused a significant increase in its content in the muscles, with a higher value noted in the leg muscles. Meat of chickens fed with the higher dose of vitamin E was also characterized by a lower cholesterol level. The addition of vitamin E as well as type of the muscles had a significant effect on the fatty acid profile and values of atherogenicity (AI) and thrombogenicity (TI) indices. The recorded increase in the content of n-3 polyunsaturated fatty acids (PUFAs) and a decrease in the n-6/n-3 fatty acid ratio indicate a positive effect of vitamin E on the fatty acid profile. In turn, lower values of AI and TI in leg muscles of chickens with a high intake of vitamin E prove the beneficial fatty acid profile in these muscles upon such supplementation.

A total of 42 barrows weighing 19.8 ± 1.22 kg were fed seven diets to give six replicates per treatment. The experiment was conducted in a factorial arrangement with factors being (1) two canola meals (CM) types and (2) three phytase levels (0, 500, and 2500 FTU kg^-1). The basal endogenous phosphorus (P) losses and standardized total tract digestibility (STTD) was calculated using the P-free method. There was no effect of CM types on feed intake and fecal P output, but an interaction effect was observed for P intake (CM × phytase; P < 0.05). Supplementation of phytase (2500 FTU kg^-1) reduced (P < 0.001) fecal P output (g d^-1), and the output was reduced by 58% in Brassica napus black (BNB) and 64% in Brassica juncea yellow (BJY) diets. Supplementation of phytase improved (P < 0.001) both apparent total tract digestibility (ATTD) and STTD of P in both BNB and BJY, regardless of dose. The basal endogenous P loss (EPL) was determined to be 111.28 ± 35.09 mg kg^-1 of dry matter intake (DMI). There was no further improvement in STTD of P when phytase was increased from 500 to 2500 FTU kg^-1 in both CM types. The ATTD of calcium (Ca) was increased (P < 0.001) in BNB and BJY when phytase was supplemented at 500 and 2500 FTU kg^-1. The results, therefore, indicate that supplementation of phytase at 500 FTU kg^-1 improved both ATTD and STTD of P in two CM types, but a super dose of 2500 FTU kg^-1 had no additional benefit. Similarly, ATTD of Ca was increased when phytase was supplemented at 500 FTU kg^-1 without further improvement at 2500 FTU kg^-1.

Whole rice bran (WRB) was added to Japanese quail feed, and the effects of stabilization of the WRB with organic acids and its storage for different amounts of time were evaluated with respect to performance and egg quality, centesimal composition, and sensory characteristics. We used 150 90-d-old Japanese quails. We had a 5 × 2 factorial scheme of five storage periods (0, 30, 60, 90, and 120 d), with and without organic acid treatment. To replace corn, 20% WRB was added to the diet. Feed consumption increased for up to 71 d of bran storage (P = 0.01) and then subsequently decreased. An increasing quadratic relationship was observed between egg mass and storage time for up to 71 d of storage (P = 0.03); egg mass then decreased thereafter. The specific gravity of the eggs produced by birds that received treated WRB at 0 d of storage was greater than those of eggs produced by birds that received treated WRB after 90 d of storage. In conclusion, the organic acid treatment maintained the quality of the WRB over up to 120 d of storage and could comprise 20% of the laying quails' diet without affecting growth performance, egg quality, bromatological composition, or the sensory attributes of the eggs.

The aims of the study were to: (1) investigate factors affecting calf birth weight (CBW) and gestation length (GL); and (2) determine risk factors associated with dystocia in 1019 Holstein heifers and cows in a tie stall dairy herd. Calf sex (CS), sire, and GL were related to CBW in primiparous and multiparous cows, with no significant effect of season, age of primiparous cows (AGE) or days in milk (DIM) of multiparous cows at conception. Gestation length was affected by CS in primiparous, with a shorter GL for female versus male calves (275.7 ± 0.5 vs. 278.3 ± 0.4; P < 0.001). Also, GL was shorter in multiparous cows that calved during the cold season compared to those that calved in mild or warm seasons. Primiparous cows had a higher prevalence of dystocia compared to multiparous cows (32.1 vs. 8.5%; P < 0.001), but it was highly variable depending on sire (24.4%-60.0% for primiparous and 0%-21.1% for multiparous). In conclusion, CS, sire, and GL were related to CBW, whereas GL was affected by CS in primiparous and season in multiparous. The CBW was the major factor affecting dystocia in primiparous cows, whereas sire and twin pregnancies were factors related to dystocia in both primiparous and multiparous cows.

Canadian beef is quality graded to characterize the potential eating quality of the cooked product. Instrumental meat quality characteristics of 48 m. longissimus thoracis (LT, rib eye) from four Canadian beef grades (Canada A, AA, AAA, and Prime, n = 12) before and after an additional 14-d aging were compared using a split plot design with grade, aging, and their interaction as fixed sources of variation. Mean percentage intramuscular fat was greatest in Canada Prime muscle and least in Canada A and AA muscles (P < 0.0001), whereas mean percentage drip loss was lower in Canada Prime muscle than in muscle from all other grades (P = 0.0348). Canada Prime and AAA muscles were redder and yellower than muscles from other grades even after aging (P < 0.03), which may be associated with increased fat content and indicative of accelerated myoglobin oxidation and increased myoglobin oxygenation. Shear force was not different among the Canada grades, although the differences between Canada AA cooked beef LT and that of Canada Prime and AAA carcasses approached significance (P = 0.0993). Results indicated that Canada quality grades did not differentiate beef on cooked product tenderness, substantiating that muscle compositional characteristics alone define beef grade advantages.

The aim of this study was to investigate the effects of dietary conjugated linoleic acid (CLA) on the cytotoxicity of peripheral blood lymphocytes in piglets. A total of forty 21-d-old Duroc × Large White × Landrace weaned piglets were randomly allocated into two treatment groups and fed with control diet or 2% CLA diet for 6 weeks. Dietary CLA significantly increased the number of peripheral blood CD8 T lymphocytes (P < 0.05). Compared with the control group, cytotoxicity, perforin, and granzyme B mRNA expression of peripheral blood lymphocytes in piglets from the CLA-supplementation group increased by 29.98% (P < 0.01), 32.87% (P < 0.01), and 43.36% (P < 0.05), respectively. The piglets on the CLA diet had higher mRNA expression of Lck and Fyn in peripheral blood lymphocytes (P < 0.05). The content of C16:0 significantly increased in piglets from the dietary CLA-supplementation group (P < 0.05), whereas the content of C18:1 significantly decreased (P < 0.05). The CLA content of peripheral blood lymphocytes in piglets in the CLA diet group ranged up to 0.24% (P < 0.01). These results suggest that dietary CLA enhanced cytotoxicity of peripheral blood lymphocytes perhaps through changing its fatty acid composition.

Two trials were conducted to study the effect of feeding barley silage on the behaviour and performance of beak-trimmed laying hens. In each trial, 20 hens and 2 roosters were housed in each of eight group-housed enriched cages, with four cages provided a laying hen diet and four cages additionally given free access to barley silage. Egg production and quality, and hen behaviour were assessed throughout the trials (Trial One 20-30 weeks; Trial Two 19-28 weeks). Data were analysed using Proc Mixed of SAS 9.4 and differences were significant when P ≤ 0.05. Silage-fed hens consumed 41 (13.9 g DM) and 50 (13.5 g DM) grams of silage per hen per day in Trials One and Two, respectively, while consuming less layer diet. Birds fed silage spent less time expressing aggressive and feather-pecking behaviours and in nest boxes, and more time feeding than control birds. Egg production, egg quality, and bird weight were not affected by treatment; yolk colour was darker for the silage treatment. Feathering quality was improved in silage-fed birds compared to control birds. It was concluded that providing hens with access to barley silage can improve welfare indicators without negatively affecting the egg production and egg quality.

DNA mutations within genes associated with melanogenesis can affect melanin production, leading to dyschromias. Genes that are involved in synthesis of melatonin and may affect the color of skin are melanocortin 1 receptor (MC1R), agouti locus (ASIP), and tyrosinase-related protein-2 (TYRP2). In this study, SNP identification within ASIP, MC1R, and TYRP2 gene fragments in wild and farmed foxes (Vulpes vulpes) was performed. Nine mutations in the ASIP gene which allowed us to distinguish seven SNP profiles, fourteen mutations and five SNP profiles in the MC1R gene, and seven SNP profiles based on four polymorphic nucleotides in the TYRP2 gene were detected. Analyses of obtained profiles indicate that ASIP did not undergo mutations in the wild, and significant variability of SNP profiles was found for TYRP2, with specific haplotypes noted for farm foxes and American and European wild foxes.

The aim of this study was to determine the growth performance and carcass characteristics of growing-finishing pigs fed diets formulated on net energy (NE) basis with or without exogenous enzyme supplementation. Twenty-four pigs with an initial body weight (BW) of 25 kg were randomly allotted one of three treatments; a barley-based control diet formulated on a digestible energy (DE) basis (Diet A), control diet formulated on an NE basis (Diet B), and Diet B multicarbohydrase enzyme (Diet C). Pigs were offered their respective diets in a 3-phase feeding program. Individual pig BW and feed disappearance were monitored once every 2 wk. Pigs were slaughtered when they reached 100 kg BW to determine carcass characteristics. During phase 1, an improvement (P = 0.02) in feed intake was observed in pigs fed Diet C compared with Diet B. In phase 3, pigs fed Diet B showed improvement in daily gain (P = 0.02) and feed efficiency (P = 0.05) compared with Diet A. Overall, when compared with control diet, pigs fed Diet B showed significant improvement in daily gain (P = 0.05) and feed efficiency (P = 0.01). In conclusion, the results indicate a better growth performance with diets formulated using the NE system. Moreover, enzyme supplementation had no effect on the overall performance of pigs.

The diverse nature of beef production was captured by establishing a farm typology based on an extensive survey of 1005 Canadian farms in 2011. The survey provided information on the type of operation, cattle numbers, feed storage and management, manure management, land use, producer demographics and attitudes to risk, and technology adoption. Principal component analysis and cluster analysis were used to understand the relationships among variables and to statistically identify farm types. A total of 41 diagnostic variables from 133 survey questions were used to define 16 principal components explaining 68% of the variation. Cluster analysis yielded eight major clusters as distinct farm types. The largest number of farms (37%) was grouped as small-scale, part-time cow-calf operations. Mixed operations (crop-beef) were next most frequent (22%), followed by large cow-calf backgrounding (18%) and diversified cow-calf operations that included crop-beef mixed operations as well as off-farm activities (11%). Cow-calf operations that finished calves comprised 8% of the total farms surveyed. Extensive cow-calf backgrounding operations, large backgrounding/finishing operations, and large finishing operations represented the remaining 3% of the farms. The typology not only provides a strategy by which the Canadian beef cattle industry can be characterized, but also improves understanding of the diversity of farm management practices to help develop policies and beneficial management practices.

Muscovy duck (Cairina moschata) is characterized by broodiness, and egg-laying traits in this species should be of primary concern. As a member of the IGF superfamily of proteins, insulin-like growth factor-1 (IGF-1) is an important positive regulator of growth and gonad development in vertebrates. To explore the effect of IGF-1 on the growth hormone/insulin-like growth factors (GH/IGF) axis, we cloned and characterized IGF-1 of Muscovy duck. The expression level of IGF-1 was widely expressed in all the tested tissues, and the highest level was detected in the liver. In laying ducks, IGF-1 expression levels in the hypothalamus, pituitary, and ovary were very significantly higher (P < 0.01) than that in the tissues of nesting duck, and IGF-1 expression levels in the heart and liver were significantly higher (P < 0.05) than that in nesting duck tissues. Furthermore, a single nucleotide polymorphism (SNP) (A/G) was found and significantly (P < 0.05) associated with age at first egg and egg number at 300 d. This study provides the first evidence that IGF-1 promote egg-laying traits of Muscovy duck through two axes, involving GH/IGF and hypothalamic-pituitary-gonadal axes. These findings enrich the information of IGF-1 of Muscovy duck and demonstrate for the first time the ability of IGF-1 to promote reproduction, indicating that IGF-1 could be used as an important marker gene.

The aim of the study was to determine the number and shape of nucleoli during meiosis in cells of the domestic horse. In addition, the level of global DNA methylation was determined using a quantitative technique for measuring the relative level of DNA methylation, modelled on an immunoenzymatic assay. The research was carried out on stallions belonging to two age groups (2 and 7 yr). In the cells of the 2-yr-old animals, the nucleoli were mainly of a regular shape and no fragmented nucleoli were observed. The cells of the 7-yr-old horses had a small percentage of regularly shaped nucleoli, and nucleoli with a fragmented structure were present. The study provides a basis for further research on epigenetic mechanisms in horses.

Application of long-chain alcohols (LCOH) and long-chain fatty acids (LCFA) combined with alkanes as faecal markers to estimate dry matter intake (DMI) and dry matter digestibility (DMD) of equines and cattle was assessed. Six crossbred mares, randomly divided in two groups (H1 and H2), and three nonlactating cows (C) were housed in individual stalls. Groups H1 and C were fed on a diet of ryegrass (Lolium perenne, 0.7) and heather (Erica spp., Calluna vulgaris, 0.3), and H2 received ryegrass (0.4), heather (0.3) and gorse (Ulex gallii, 0.3). Digestibility was estimated using LCOH (C₂₈-OH and C₃₀-OH) and LCFA (C₂₈-FA, C₃₀-FA, and C₃₂-FA) as internal markers. For DMI estimation, animals received daily a paper pellet containing C₂₄, C₃₂, and C₃₆n-alkanes. Intake was estimated from the faecal ratio of naturally occurring LCOH, LCFA, and dosed n-alkanes, and was compared with the known DMI values. In horses, all markers provided accurate estimates of DMD. Similarly, LCOH provided accurate estimates of DMD in cattle, whereas LCFA underestimated it (P < 0.05). Intake estimates were affected (P < 0.05) by the marker pair used in calculations. In general, the C₂₄:C₂₄-FA pair provided the most accurate DMI estimates. Results suggest the usefulness of combining epicuticular compounds as faecal markers to estimate DMI, DMD and diet composition of horses and cattle grazing grass-heathland communities, simultaneously.

Two studies were conducted to evaluate the provision of high-lipid high-fibre byproduct pellets when used as a partial replacement (60% in Study 1 and 30% in Study 2; HLP) for barley grain and canola meal in finishing diets (BAR). The HLP was fed for the last 49, 98, or 147 d (HLP49, HLP98, and HLP147, respectively) in Study 1, and for the last 60 or 120 d in Study 2 (HLP60 and HLP120, respectively) or the last 60 d with additional canola oil (HLP60CO). The statistical model included the fixed effects of diet, period, and the interaction. Steers fed BAR147 had the greatest average daily gain (ADG) (P < 0.01) and G:F (P = 0.01). The HLP147 had the greatest dry matter intake (DMI) during the first 49 d but least during the last 49 d of the finishing phase (treatment × period; P < 0.01). Hot carcass weight for BAR147 and HLP49 were the heaviest (P = 0.04). In Study 2, DMI and ADG were not affected (P > 0.05), but hot carcass weight was greater for BAR120 and HLP60 than HLP120 and HLP60CO. Inclusion of high-fibre high-lipid byproduct pellets in the latter part of the finishing period may improve carcass yield grade without affecting ADG and G:F.

Effect of carbohydrases and phytase supplementation on growth performance, nutrient utilization and gut health of nursery pigs was evaluated. Pigs were blocked by body weight (BW) and sex and allocated to four treatments. Treatments were a positive control (PC), a negative control (NC) deficient in metabolizable energy (ME), crude protein (CP), Ca, and non-phytate P (nPP), NC plus Rovabio® Max AP enzyme mix, at 0.05 and 0.075 g kg^-1. Apparent total tract digestibility (ATTD) was determined in faecal samples. Apparent ileal digestibility (AID) was determined in ileal digesta samples collected after euthanasia. Lower final BW and average daily gain (ADG) (P < 0.05) were observed in NC compared with PC. Enzyme at 0.05 g kg^-1 increased (P < 0.05) BW on d 14 and d 41, respectively, and also increased ADG. Enzyme at 0.075 g kg^-1 increased BW on d 14 and ADG on d 0 to d 14 (P < 0.05). Feed efficiency [gain to feed ratio (G:F)] was greater (P < 0.05) in PC than NC from d 15 to d 41 and from d 0 to d 41. No difference in G:F was observed with enzyme supplementation. Higher (P < 0.05) serum Ca and bone ash were observed in PC than NC. Enzyme increased the ATTD of Ca and P (P < 0.05) compared with NC.

The objective of this paper was to evaluate changes in management practices of beef cattle from 2005 to 2011. Large nationwide surveys of husbandry practices in the beef industry were conducted to represent management practices used in 2005 and 2011 across Canadian Ecoregions. The two surveys attempted to similarly represent operation types (cow-calf, backgrounding, and finishing) and size. Several statistically significant changes in management practices from 2005 to 2011 were observed: in non-feedlot operations, these included more operations with >50% legume in perennial forage, less N fertilization applied to forages, increased winter grazing of cows, and higher quality feed used as a supplement to grazing. In feedlots, there were more shelter structures, less feeding of grain and more of high-legume forages, and more frequent removal of manure. Several practices remained constant in the two surveys including use of covers for storing hay, frequency of harvesting forage, time of barn and feedlot cleanout, manure storage and practices relating to manure incorporation into soil. The large increase in use of winter grazing on the Prairies as well as in eastern Canada documented here is an important change in the industry that has both economic and environmental implications.