Fallow deer meat (venison) is a new product acquired from farm-raised and wild animals. Nowadays, people are more concerned about their health, and therefore, they search for high quality, “healthy” products. Farming of fallow deer is ecological and friendly to the environment. Animals are kept in pasture conditions which resemble their natural environment. Venison is considered healthy meat, low in fat and cholesterol, and high in protein. The aim of this review was to gather and discuss the available literature on factors influencing the quality of venison such as sex, age, slaughter method and housing system, feeding, hanging method, animal condition, muscle type, and ageing of meat, considering both farm-raised and wild fallow deer.

Multiple factors affect the risk of broiler injury and mortality during loading, transport, and lairage. These include the physical and pathophysiological condition of broilers before loading and the type of handling procedures used. The external environmental conditions have a major influence on the risk of mortality. Combinations of high stocking density, insufficient ventilation, and high temperature and humidity pose a risk of hyperthermia. Combinations of wet conditions, cold temperature, and air movement, increase the risk of hypothermia. However, protection from cold and wet conditions involves the use of side protection that restricts the trailer ventilation that can cause heat and moisture to build up in the load, increasing the risk of hyperthermia. Modular systems reduce the risk of injury and facilitate unloading into a lairage where temperature and ventilation can be controlled. The duration of loading, transport, and lairage increases the mortality risk. Deaths can occur during each stage and interactions between the duration of the preslaughter stages, the thermal environment, fasting, ill-health, and injury can reduce the physiological capacity of the birds to maintain homoeostasis resulting in exhaustion and death. Quality control and action on the identified risk factors should reduce injury and mortality during preslaughter handling and transport.

This study evaluated the efficacy of a matrix-coated organic acids and medium-chain fatty acids blend (MCOFA) in growing pigs. Ninety six pigs [(Yorkshire × Landrace) × Duroc] with an average body weight (BW) of 47.71 ± 3.73 kg were used in a 6 wk experiment. Pigs were allotted to diets containing 0 or 2 g kg^-1 of MCOFA, and 0 or 2.5 g kg^-1 of antibiotic growth promoters (AGP) according to a 2 × 2 factorial arrangement of treatments. Pigs fed diets supplemented with MCOFA had improved growth efficiency compared with those fed a diet without MCOFA (P < 0.05). Pigs receiving the diets supplemented with both AGP and MCOFA had higher apparent total tract digestibility of crude protein, dry matter, fat, and gross energy (P < 0.05). Pigs fed AGP × MCOFA diet had increased serum urea nitrogen (P < 0.05). Pigs fed diets supplemented with AGP had reduced fecal ammonia (NH₃) gas emissions compared with those fed without AGP (P < 0.05). Moreover, pigs fed diets supplemented with MCOFA had reduced fecal NH₃ and acetic acid gas emissions compared with those fed without MCOFA (P < 0.05). In conclusion, dietary supplementation with MCOFA improved performance in growing pigs.

We examined the relationship of Hanwoo (Bos taurus coreana) beef sensory attributes with intramuscular fat (IMF), Warner–Bratzler shear force (WBSF), and other environmental factors. Samples of 458 beef carcasses including 32 cows, 34 steers, and 392 bulls were analyzed for IMF percentage, WBSF, and important sensory attributes (tenderness, juiciness, and flavour-likeness). Results revealed that steer beef had significantly higher scores for all three sensory attributes than beef from cows and bulls. While juiciness and flavour-likeness differed (p < 0.05) among bulls and cows, they were not associated an effect on tenderness. All sensory attributes and WBSF were correlated (p < 0.001) with IMF; the highest correlation coefficient was for tenderness (0.55) while a negative coefficient (-0.39) was found for WBSF. The influence of IMF level on all three sensory attributes of bull beef significantly increased with IMF level. All sensory attributes of steer and bull beef showed negative relationship (p < 0.01) with WBSF, where highest correlation coefficient (-0.8) found for steer beef tenderness. Furthermore, tenderness and flavour-likeness showed lower ranks for meats slaughtered during the winter season; however, juiciness did not differ (p < 0.05) among seasons.

Meat quality characteristics have been measured on fresh and previously frozen meat as part of genetic studies, but freezing of meat may alter its quality characteristics and, therefore, the relationships between genetic components and meat quality measurements. Pork color, pH, and drip loss measurements performed on longissimus dorsi from the carcasses of 2027 crossbred commercial pigs when either fresh or thawed after frozen storage were used to estimate genetic parameters using a bivariate animal model in ASReml. Meat quality traits measured before and after freezing and thawing were significantly (P < 0.0001) different from each other and intramuscular crude fat content exerted a large effect on the magnitude of change in L* (lightness) and b* (yellowness). Meat quality measurements of fresh pork were moderately to highly heritable except for b* and pH, with heritability estimates for L*, pH, and drip loss greater when measured on fresh rather than frozen-thawed samples. Considering heritability and genetic correlation results, we concluded that whilst either fresh or frozen-thawed pork samples can be used for fresh pork L*, a* (redness), and b* measurements, pH, and possibly drip loss should be measured in fresh pork samples rather than in those that have been frozen-thawed during genetic selection for fresh pork quality.

The objective of the study was to measure the effect of wheat market class and cultivar on starch digestibility using an in vitro model that mimics the chicken digestive tract and relate it to grain characteristics. The study evaluated 18 wheat cultivars from eight western Canadian wheat classes and, each cultivar was replicated four times. Samples were subjected to gastric and small intestine (SI) digestion phases and each sample was assayed in triplicate; glucose release was measured in SI phase. Starch granule distribution, amylose, total starch, crude protein (CP), ash, and non-starch polysaccharides (NSP) were analyzed in all wheat samples. Small intestinal phase times of 15, 60, and 120 min were chosen to approximate digestion in the terminal duodenum, jejunum, and ileum. Starch digestibility of wheat classes ranged as follows: 15 min — 33.1% to 49.1%, 60 min — 80.2% to 93.3%, and 120 min — 92.4% to 97.6%. Starch digestibility positively correlated with CP, ash, NSP, and proportion of large granules, whereas it negatively correlated with total starch, and proportion of small and medium granules. In conclusion, market class and cultivar of western Canadian wheat affects both rate and extent of starch digestibility and it is related to various grain characteristics.

The objective of this study was to determine the effects of live yeast (LY) or yeast derivatives (YD) on gas production (GP), dry matter (DM) disappearance (DMD), fermentation characteristics, and microbial profiles in batch culture. The study was a completely randomized design with a factorial arrangement: 2 media pH × 5 yeasts × 4 dosages. An additional treatment of monensin (Mon) was added as a positive control for each pH level. Media pH was low (5.8) and high (6.5); the yeasts were three LY (LY1-3) and two YD (YD4-5); and doses were 0, 4 × 10⁶, 8 × 10⁶, and 1.6 × 10⁷ cfu mL^-1 for LY and 0, 15, 30, and 60 mg bottle^-1 for YD. Substrate consisted of 10% silage and 90% concentrate (DM basis) and samples were incubated for 24 h. Media pH of 6.5 vs. 5.8, increased (P < 0.01) GP, DMD, and volatile fatty acid (VFA) concentrations but decreased (P < 0.01) NH₃-N concentration and copy numbers of Ruminococcus albus, Ruminococcus flavefaciens, and Selenomonas ruminantium. Increasing dose of LY2 linearly (P < 0.05) increased DMD. Total VFA concentration was greater with LY2 (P < 0.01) than LY3 and YD5 at pH 6.5. Overall, adding yeast products improved (P < 0.05) DMD at pH 5.8, and increased VFA concentration compared with Mon. These results indicate that in vitro GP and DMD of a high-grain diet varied with source and dose of yeast supplementation. Some yeast products have the potential to improve fermentation of feedlot diets when supplemented at appropriate doses.

A total of 180 crossbred pigs [(Landrace × Yorkshire) × Duroc] with an average body weight of 22.61 ± 1.23 kg were used in an 18 wk study to determine the effect of protease and bromelain in low-protein diets in grower–finisher pigs. Dietary treatments included: T1, basal diet treatment; T2, low-protein treatment; T3 (T2 0.2 g kg^-1 protease); and T4 (T2 0.3 g kg^-1 bromelain). Pigs fed protease- and bromelain-supplemented diets increased average daily gain and gain to feed ratio at week 18, dry matter and nitrogen digestibility at week 6, as well as energy digestibility at week 12, compared with low-protein diet (P < 0.05). Pigs fed T3 and T4 diets led to a trend of decreased (P < 0.05) blood urine nitrogen (BUN) and creatinine concentrations at the 12th wk. A reduction of ammonia (NH₃) and hydrogen sulfide (H₂S) emission was observed in pigs fed the dietary protease and bromelain supplementation at the 6th wk (P < 0.05). There was no effect on all parameters between the protease and bromelain supplementation treatments. In conclusion, supplementation of protease and bromelain to low-protein diet enhanced growth performance, nutrient digestibility, and reduced NH₃ and H₂S in growing–finishing pigs.

The considerable animal-to-animal variation in microbial profiles is a challenge in elucidating the role of gut microbiota in host metabolism. The main purpose of this study was, therefore, to develop a pig model with reduced animal-to-animal variation in gut microbial profile. Twelve piglets from four sows were reared conventionally and 12 piglets from four sows were reared artificially in high efficiency particulate air (HEPA) filtered isolators. All isolator-reared piglets were given an artificial colostrum formula containing the combined fecal material from all eight sows. All piglets were killed at 21 d of age and intestinal contents subjected to 16s rRNA gene-based terminal restriction fragment length polymorphism (T-RFLP) profiling. Resulting T-RFLP profiles clustered into two distinct groups representing the two treatment groups. Furthermore, Bray–Curtis dissimilarity distance values and Dice similarity indices showed reduced beta diversity in isolator-reared pigs indicating animal-to-animal variation was reduced in isolator-reared compared to conventional piglets. However, surprisingly, increased alpha diversity was observed in isolator-reared piglets compared with conventional piglets. In conclusion, the study demonstrated that rearing of piglets under conditions of controlled environment reduced animal-to-animal variation in the hindgut microbiota while paradoxically increasing within animal microbial diversity. Isolator rearing may be useful as a model to improve detection of treatment effects on gut microbiota.

The present study was aimed to evaluate the use of increasing levels of castor bean cake detoxified with calcium hydroxide in diets for pigs at growing and finishing phases on performance, diet digestibility, weight of digestive organs, carcass traits, and economic evaluation. A total of 40 barrows with 44.59 ± 5.16 kg initial weight were fed diets with 0%, 7%, 14%, and 21% detoxified castor bean cake. Increasing levels of detoxified castor bean cake in diets resulted in linear worsening in the average daily gain of the pigs. Dry matter, crude protein, and gross energy digestibilities were adversely affected by the levels of detoxified castor bean cake. A linear decrease in loin eye area and ham yield was detected depending on detoxified castor bean cake inclusion levels. Although the detoxified castor bean cake had no effect on the assessed economic variables, its use in diets for growing and finishing pigs is not recommended.

This study was conducted to determine the standardized ileal digestible amino acid (AA) and nitrogen-corrected apparent metabolizable energy (AME_n) contents of a high-protein sunflower meal (HiSFM) fed to broiler chicks. A total of 144 broiler chicks were randomly allotted to one of three dietary treatments with eight replicate cages of six birds. Birds were fed the experimental diets from day 14 to day 21 of age. The results showed that gross energy, crude protein, nonphytate phosphorus, lysine (Lys), methionine (Met), threonine (Thr), and total nonstarch polysaccharide contents of HiSFM were 4178 kcal kg^-1, 48.7%, 0.86%, 1.59%, 0.75%, 1.45%, and 14.6%, as-fed basis, respectively. The standardized ileal digestible Lys, Met, and Thr of HiSFM were 12.6, 6.8, and 11.6 g kg^-1 (dry matter basis), respectively. In addition, the AME_n content of HiSFM fed to broiler chicks was 1785 kcal kg^-1. In conclusion, compared with literature values for sunflower meal, HiSFM had lower standardized ileal digestibility of indispensable AA and higher AME_n content. The standardized ileal digestible AA and AME_n values of HiSFM obtained from the current study could be used when formulating poultry diets and to obtain predictable bird performance.

In this study, we aimed to identify metabolite signatures that characterize metritis prior to, during, and after the disease incidence. Blood samples were collected from 100 Holstein cows at five time points before and after parturition. Six cows that developed metritis and 20 controls were selected for metabolomics analysis in a nested case-control study. Twenty nine serum metabolites were quantified using gas chromatography–mass spectroscopy. Results showed that similar panels of metabolites differentiated pre-metritic and control cows at 8 and 4 wk prepartum. The top most important metabolites that differentiated the two groups of cows at 8 wk prepartum were oxalate, ornithine, pyroglutamic acid, d-mannose, and glutamic acid, and at 4 wk prepartum were ornithine, pyroglutamic acid, d-mannose, glutamic acid, and phosphoric acid, suggesting their potential use as risk biomarkers for metritis. Area under the curve with values of 1.0 and 0.969 at 8 and 4 wk, respectively, indicated that those panels of metabolites have a very high sensitivity and specificity to be used as risk biomarkers for metritis. Overall, results showed that specific serum metabolite signatures can be used to screen cows for susceptibility to metritis during the dry off period, and to better understand the etiopathobiology of the disease.

Effects of a lipid-coated zinc oxide (ZnO) Shield Zn^® (SZ) vs. ZnO were evaluated. Forty 25-d-old weanling pigs were fed a nursery diet supplemented with 100 mg kg^-1 Zn with ZnO (ZnO-100), ZnO-2500, SZ-100, -200, or -400. All piglets were challenged orally with 5 × 10⁸ colony-forming units of enterotoxigenic Escherichia coli K88 on day 7 and euthanized on day 14. The fecal consistency score (FCS) was less for the SZ group vs. ZnO-100 (P < 0.05). The intestinal villus height:crypt depth ratio and goblet cell density were greater for the SZ group vs. ZnO-100. By regression analyses, SZ-100 to -200 and SZ-300 to -400 were comparable to ZnO-2500 in the FCS and intestinal variables, respectively. The jejunal mucosal mRNA level did not differ between the SZ group and either ZnO group in insulin-like growth factor-I and multiple structural proteins and cytokines including zonula occludens protein (ZO) 1 and interleukin (IL) 10 except for lower ZO-1 and IL-10 mRNA levels for the SZ group than for ZnO-2500 and ZnO-100, respectively. The ZO-1 mRNA level regressed positively on the supplemental SZ concentration. Results suggest that SZ play a role in epithelial barrier function and inflammation by modulating the expression of ZO-1 and IL-10.

A total of 246 357 measurements of birth (BW) and probe (PW) weights of purebred Yorkshire and Landrace pigs were used to compare the fitting of two alternate models including either common-litter effect or cross-fostering group effect to account for common environmental variation. PW, is a live ultrasonic weight measurement taken when the pigs are 100 ± 30 kg, following national standards. The common-litter effect was defined as piglets born into the same litter, and the group effect was used to account for cross-fostering, and defined as the effect common to piglets raised by the same nurse-sow, regardless of whether that piglet was born into that litter or not. It was found that the cross-fostering group explained 5% more environmental variation in BW when compared with the common-litter effect, indication that BW is a criterion in cross-fostering. Cross-fostering also explained 1% more environmental variation in PW in both the Yorkshire and Landrace. When the cross-fostering group effect was included in place of the common-litter effect, the direct and maternal genetic heritability estimates were similar, but residual variances were reduced. This study advanced the understanding of the effects of cross-fostering on PW, its association with BW and its implications in modern pig breeding programs.

The objective of this study was to investigate the effect of carcass weight on chemical characteristics and fatty acid composition of m. longissimus dorsi and m. semimembranosus of European wild boar (Sus scrofa scrofa) meat. The research was carried out on 50 wild boars shot during four battues at hunting reserves in the central part of Poland. The carcasses were divided into three groups: ≤30 kg (G1), 31–45 kg (G2), and >45 kg (G3). The protein concentration increased with carcass weight (P ≤ 0.01). The concentration of saturated fatty acids was influenced by carcass weight and was higher when weight increased. A significantly higher level of monounsaturated fatty acids (MUFA) was found in the carcasses of wild boars from G3 compared with G1 group (P ≤ 0.01). The content of C18:2n-6 was significantly lower in the lightest carcasses compared with the heaviest (P ≤ 0.05). Statistical analysis confirmed that carcasses weight significantly affected polyunsaturated fatty acids (PUFA) n-3 content. The lightest carcasses contained a significantly higher concentration of PUFA n-3 (P ≤ 0.01). In addition, in G3, the amount of C22:5n-3 was two-fold lower (P ≤ 0.01) than in G1. The research has shown that the meat quality was affected by the carcass weight, resulting better parameters in the lighter than in the heavier carcasses. Therefore, most of the culled wild boars should be squeakers and young boars after the first year of life.

The statistical methods used in the genetic evaluations are a key component of the process and can be best compared by using simulated data. The latter is especially true in grazing beef cattle production systems, where the number of proven bulls with highly reliable estimated breeding values is limited to allow for a trustworthy validation of genomic predictions. Therefore, we simulated data for 4980 beef cattle aiming to compare single-step genomic best linear unbiased prediction (ssGBLUP), which simultaneously incorporates pedigree, phenotypic, and genomic data into genomic evaluations, and two-step GBLUP (tsGBLUP) procedures and genomic estimated breeding values (GEBVs) blending methods. The greatest increases in GEBV accuracies compared with the parents’ average estimated breeding values (EBV_PA) were 0.364 and 0.341 for ssGBLUP and tsGBLUP, respectively. Direct genomic value and GEBV accuracies when using ssGBLUP and tsGBLUP procedures were similar, except for the GEBV accuracies using Hayes’ blending method in tsGBLUP. There was no significant or slight bias in genomic predictions from ssGBLUP or tsGBLUP (using VanRaden’s blending method), indicating that these predictions are on the same scale compared with the true breeding values. Overall, genetic evaluations including genomic information resulted in gains in accuracy >100% compared with the EBV_PA. In addition, there were no significant differences between the selected animals (10% males and 50% females) by using ssGBLUP or tsGBLUP.

The aims of this study were to investigate the effect of Saccharomyces cerevisiae var. boulardii CNCM I-1079 (SCB) or Lactobacillus acidophilus BT1386 (LA) on (1) innate immune response, (2) markers of acute-phase reaction, and (3) immune gene expression of rumen and ileum tissues of Holstein calves. Forty eight calves (?5 d old) were randomly allocated to four treatments as follows: (1) control (CTRL) fed milk replacer followed by starter feed, (2) CTRL supplemented with SCB in milk and feed, (3) CTRL supplemented with LA in milk and feed, and (4) CTRL supplemented with antibiotics (ATB; chlortetracycline and neomycin in milk, and chlortetracycline in feed). Tumor necrosis factor α (TNF-α) decreased (P < 0.05) on day 66 (post-weaning) for the ATB-treated calves. There were no treatment effects on production of interferon γ (IFN-γ) and interleukin 6 (IL-6) proteins and on expression of TLR4, TLR6, TLR9, TLR10, CLDN3, MUC1, and MUC20 genes. Calves fed SCB or LA had a greater (P < 0.05) oxidative burst at weaning (day 53) compared with CTRL. Oxidative burst was also greater (P < 0.05) after weaning (day 59 and day 87) for SCB-fed calves. Calves fed SCB and ATB had higher (P < 0.05) phagocytosis activity during weaning (day 47) compared with CTRL. The concentration of serum amyloid A2 (SAA2) increased (P < 0.05) in SCB- and LA-fed calves (day 53), whereas the concentration of C-reactive protein (CRP) increased (P < 0.05) in SCB-fed calves during weaning as compared with CTRL. Our results suggest that SCB could improve innate immune response (oxidative burst and phagocytosis) and markers of acute-phase reaction (CRP and SAA2), especially during critical periods like weaning.

Level of feed intake did not affect the apparent total tract digestibility (ATTD) of calcium (Ca) and phosphorus (P) in gestating sows. Gestating sows had reduced ATTD of Ca and P compared with growing gilts. Formulating diets for sows using values obtained from growing pigs, therefore, may result in inaccuracies.

Two in vitro batch culture experiments were conducted to obtain a total of 1811 paired data for headspace gas pressure (G_P; PSI) and volume (G_V; mL). Data were used to generate and evaluate an equation to estimate G_V from G_P under our laboratory conditions (G_V = 4.7047 G_P 0.0512 G_P²).

Physical and chemical body composition of gilts and parity 3 sows were used to determine current prediction equation accuracy and propose alternative prediction equations that incorporate additional variables. Longissimus dorsi muscle depth and parity can be combined with body weight and backfat to improve gilt and sow body composition prediction.

Daily urinary nitrogen excretion (UN, g d^-1) was quantified in gestating gilts during 5 d nitrogen (N) balance periods. Abbreviated collection days (<22 h) can be used in N balance calculations when extrapolated to reflect a complete 24 h collection. Variation in daily UN did not affect overall N retention calculations.