Pharyngodon frenatusi (Nematoda: Pharyngodonidae) is reported from Brooks’ house gecko, Hemidactylus brookii, and flat-tailed house gecko, Hemidactylus platyurus, from Kirtipur, Nepal. Fifteen samples of H. brookii and 10 H. platyurus were collected in 2014 and examined for helminth parasites. Pharyngodon frenatusi was found in great numbers in the intestines of both hosts. The prevalence of P. frenatusi in H. brookii was 73%, and in H. platyurus, it was 80%. This is the first report, to our knowledge of P. frenatusi reported in house geckoes of Nepal. Hemidactylus platyurus represents a new host record for P. frenatusi.