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4 October 2013 Interspecific introgression of male sterility from tetraploid oilseed Brassica napus to diploid vegetable B. rapa through hybridisation and backcrossing
Zhengjie Wan, Yuanbao Tan, Minhui Shi, Yuejin Xu, Nader Aryamanesh, Guijun Yan
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Abstract

Interspecific F1 hybrids were obtained from a cross between a male sterile Brassica napus (2n = 4x = 38, AA (20) and CC (18) genomes) and an inbreeding line B. rapa (Purple Cai-Tai inbred line 9418, 2n = 2x = 20, AA (20) genome) to introgress male sterility from a tetraploid into a diploid through backcrossing. The morphological characteristics of F1 plants were more like the female parent B. napus and segregated considerably in BC1 when backcrossed to the recurrent parent Purple Cai-Tai. The progeny became stable and more similar to Purple Cai-Tai by BC4. Most C genome chromosomes were found to be eliminated, based on cytogenetic analysis. The majority of chromosomes were eliminated at very early backcross stages, with only 20–26 chromosomes in BC1 plants, and some chromosomes were eliminated gradually with increased backcross generations. The BC4 plants were generally stable with exactly 20 chromosomes. Analysis by AFLP indicated that 49.5–68.7% of the total bands eliminated from F1 to BC4 were female parent specific, and ∼12% of B. napus bands were retained with increased backcrossing. The genetic materials controlling sterility from the female parent B. napus were introgressed successfully into the BC4 plants even though most B. napus chromosomes/genetic materials were eliminated during the backcross process.

© CSIRO 2013
Zhengjie Wan, Yuanbao Tan, Minhui Shi, Yuejin Xu, Nader Aryamanesh, and Guijun Yan "Interspecific introgression of male sterility from tetraploid oilseed Brassica napus to diploid vegetable B. rapa through hybridisation and backcrossing," Crop and Pasture Science 64(7), 652-659, (4 October 2013). https://doi.org/10.1071/CP13103
Received: 28 March 2013; Accepted: 1 September 2013; Published: 4 October 2013
KEYWORDS
amplified fragment length polymorphism (AFLP)
chromosome elimination
distant hybridisation
introgression
Purple Cai-Tai
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