Twelve new species are added to the genus Ladoffa Young from Central and South America. The new species are Ladoffa dentata, L. breva, L. ancistra, L. flexa from Colombia; L. guianae, L. stenota from French Guiana; L. suttoni from Guatemala; L. reversa, L. bartletti from Costa Rica; L. lobata from Panama; and L. meiota, L. bifurca from Belize. A listing of all the species of this genus is included with the current known distribution of each species.

Reduviid species are recorded as indigenous natural enemies of tea mosquito bug (Helopeltis spp.), which is one of the major economically important pests of cashew. Rihirbus trochantericus laid eggs singly as well as in groups of up to 26 eggs in 3 to 7 clusters per female. The incubation period was 13.00 ± 0.69 days. The stadial durations of I, II, III, IV and V nymphs were 12.39 ± 1.13, 7.00 ± 0.39, 7.56 ± 0.35, 9.28 ± 0.64 and 12.78 ± 1.27 days, respectively. Adult males and females survived for 107.13 ± 2.70 and 117.9 ± 3.83 days, respectively and their sex ratio was 1: 0.7. The sequential acts of prédation as well as mating conform to those of Harpactorine reduviids. R. trochantericus exhibited Holling's type II functional response. The molecular characterization of R. trochantericus will be highly useful in confirming the identity of the species in any of its life stages.

New distributional records (45) are recorded for fishflies in West Virginia. Thirteen new county records were added for Nigronia serricornis (Say), and 18 new county records were recorded for N. fasciatus (Walker). Eight new county records were added for Neohermes concolor (Davis), while six new county records were noted for Chauliodes pectinicornis (Linnaeus). Following this investigation, the statewide distribution of fishfly species is as follows: Nigronia serricornis (45 counties), N. fasciatus (37 counties), Neohermes concolor (10 counties), Chauliodes pectinicornis (19 counties), and C. rastricornis (3 counties).

Tarter and Watkins (1974), Watkins, et al. (1975), and Dolin and Tarter (1981) have reported distributional records of fishflies for West Virginia. Tarter, et al. (1977) noted seasonal emergence patterns for fishflies in West Virginia. The main objective of this investigation is to report new county records of fishflies for West Virginia.

Three eastern genera of fishflies, Chauliodes Latreille, Neohermes Banks, and Nigronia Banks, are placed in the subfamily Chauliodinae. Generally, larvae of lentic species, C. pectinicornis (Linnaeus) and C. rastricornis Rambur, are found in logs near ponds, swamps, lakes, and oxbows, whereas the larvae of lotic species, N. fasciatus (Walker) and N. serricornis (Say), are found under rocks in streams (Tarter, et al., 1976). Larvae of Neohermes concolor (Davis) are found under leaf litter near spring seeps (Tarter, et al., 1979).

Larval and adult specimens examined for this investigation are deposited at the following institutions and agencies: West Virginia Benthological Survey (WVBS) at Marshall University, Huntington, WV; West Virginia Department of Environmental Protection (WVDEP), Charleston, WV; and West Virginia Department of Agriculture (WVDA), Charleston, WV.

Mark-release-recapture provides a technique to estimate population sizes, dispersal, survival, and demographic data. This technique was employed with the small blister beetle, Gnathium minimum, at Spring Green Prairie State Natural Area from 24 July to 16 August 2008. Of 104 marked adults, 54 were recaptured; a maximum of 34 were observed during a single survey and a maximum daily Jolly-Seber population size estimate of 59 individuals was calculated. Most individuals remained within 12 m of their original capture location, suggesting they remained within the same patch of Helianthus occidentalis flowers or an adjacent patch. The furthest movement observed was 601 m.

Seven new species of Longicauda Zhang & Wu in the subfamily Idiocerinae are described from Thailand. The new species are: L. circula, L. fasciata, L. loeiensis, L. obliqua, L. plura, L. quadrata, and L. similis.

A procedure to extract genomic DNA from small insects of Blastophaga javana Hill (Hymenoptera: Agaoninae) and Ceratosolen solmsi marchali (Hymenoptera: Agaoninae) for PCR amplification is described. We compared the amplification of both cytochrome oxidase I (COI) gene and nuclear gene (Microsatellites) for specimens with different storage times in 95% ethanol. We found that with the modified protocol, a single, clean DNA fragment from the specimens can be amplified. The PCR amplifications from specimens with shorter storage times are much better. We found that the fragment that the microsatellite (SSR) amplified from B. javana is single and stable. This method is simple, reliable, economical, needs minimal equipment and reagents, and is important in addressing the difficulty of DNA extraction from a single very small insect.