The current study aimed to determine the DNA damage; variations induced in elemental contents as well as and fatty acids profile in Galleria mellonella male adults body tissues irradiated as pupae with sterilizing and sub sterilizing doses. Data from the present study revealed that DNA damage was directly dependant upon to the irradiation dose. It was found that the free fatty acids declined in males after irradiation with the sub sterilizing dose 100Gy while the free fatty acids raised after irradiation with the sterilizing dose 260Gy when compared to the unirradiated males. The results investigated the presence of 13 elements in both normal and irradiated males. Carbon and nitrogen elements were the most dominant elements; while chloride, silicon and calcium were the least elements. Moreover, some elements increased and others decreased as a result of the irradiation. Therefore, it can be concluded that these techniques can contribute to the separation of irradiated and non-irradiated males in sterile males' programs.