At the 120-acre Jupiter Inlet Lighthouse Outstanding Natural Area (ONA) located in southeast Florida, 30 Cladonia perforata thalli were translocated in 2009. Thalli were transplanted from an area with a large C. perforata population, where sand pine fuels had accumulated and were at risk of a fire, to an area that had either been recently burned, or where no fuel treatments were planned in the near future. There was no C. perforata at or near the four transplant recipient locations, which were selected for habitat suitability and their protected locations within the ONA. The four C. perforata recipient sites have been monitored annually since 2009. Across the four transplant locations, C. perforata thalli have increased, numbering 173 in 2015 and 443 in 2020. Based on our findings, translocation of C. perforata was highly successful at the Jupiter Inlet Lighthouse ONA, with an over 14-fold increase in number of individuals in an 11-year period, assisting the colonization of new sites and buffering the lichen's vulnerable population at this location. Larger thallus size, transplant recipient site protection, and site characteristics that facilitated thalli stabilization probably contributed to this success. While habitat conservation of in situ populations is the preferred management practice for species conservation, it is encouraging to know that, with proper methodology and timing, translocation can be a tool for certain species.

Based on field collections made during the summers of 1987, 1988, and 1989, we report 159 species of lichens in 69 genera for the Jarbidge Wilderness Area (JWA) and adjacent Forest Service lands, located in the northern Great Basin, USA. Specimens were collected at 25 sites, distributed across the JWA along with two additional sites north of the wilderness boundary on Forest Service land. Collections were made from all available substrates, including rocks, bark, lignum, mosses, soil, and from the thalli of various saxicolous lichens. All basic growth forms were found, especially crustose and foliose species. Umbilicate species (Dermatocarpon spp., Rhizoplaca spp., and Umbilicaria spp.) were commonly found on rocks, while a limited number of fruticose species (Letharia spp. and Bryoria spp.) were found on bark and lignum substrates. A single, fruticose species was found on rocks (Pseudephebe minuscula). Saxicolous substrates supported the richest lichen communities, followed by corticolous and lignicolous substrates. Specifically, cottonwood spp., various conifer species, and curled leaf mountain mahogany were important bark and lignum substrates. Other taxa occurred on soil/bryophyte/detritus substrates with one species commonly collected on the thalli of various saxicolous lichens (i.e., Caloplaca epithallina). The JWA lichen flora is richer than other Nevada wilderness areas so far surveyed, with 33 putative new species records for the state of Nevada. Species richness and abundance in the JWA is likely due in part to the more mesic conditions found in the wilderness as well as its proximity to the more abundant and diverse lichen communities found in the Northern Rocky Mountains and the Pacific Northwest. Samples of two sensitive indicator species (Rhizoplaca melanophthalma and Letharia lupina) were analyzed to determine thallus concentrations of three potential air pollutants (sulfur, copper, and lead). All pollutant element concentrations were within background levels except for percent sulfur in two samples of Letharia lupina from Hummingbird Ridge (0.226%) and Emerald Lake (0.215%). Both sites are upper elevation (> 2700 m ASL) suggesting possible accumulation due to long-range transport of sulfur species from large urban areas in the Pacific Northwest.

Species lists of hornworts and liverworts with state distributions do not currently exist for Washington State. This lack of foundational knowledge frustrates current collectors and limits conservation efforts. This list was created using herbarium collections from the Consortium of Pacific Northwest Herbaria and the Consortium of North American Bryophyte Herbaria. The hornwort data reveal a very old dataset of 168 records, four accepted taxa distributed among 15 counties. The liverwort data reveal a dataset of 17,147 records, 201 accepted taxa distributed among 35 counties. Lists of Accepted Taxa and Excluded Taxa are given. Acknowledging limitations in the data, this effort will hopefully provide impetus for further collection and discussion of hornworts and liverworts in the state of Washington.

Identification of many lichens require determination of their chemical constituents. Thin-layer chromatography is a chemical method for separating out chemicals by their solubility in organic solvents. We present here a tested, simplified protocol for performing thin-layer chromatography using locally available materials and reagents, based on solvent system C of standard lichen chromatographic protocols. Adapting Solvent System C, commercial-grade reagents were substituted and the protocol modified to be performed outside of a laboratory without the need of a special license for purchasing laboratory-grade chemicals and without special handling of hazardous chemicals and waste. Commercial-grade materials and chemicals were exchanged step-wise throughout the process. Trials completed using commercial-grade materials and chemicals were successfully compared to using labgrade materials and chemicals.