Lotmaria passim Schwarz (Trypanosomatidae) is a recently described trypanosome parasite of honey bees in the continental United States, Europe, and Japan. We developed a multiplex PCR technique using PCR primers specific for L. passim or Crithidia species to distinguish L. passim from C. mellificae Langridge and McGhee. We report the presence of L. passim in Hawaii and American Samoa for the first time. More importantly, this multiplex PCR will be a useful diagnostic technique for screening honey bee samples for the presence of this pathogenic trypanosome.
How to translate text using browser tools
1 December 2016
Molecular Diagnostics of the Honey Bee Parasites Lotmaria passim and Crithidia spp. (Trypanosomatidae) Using Multiplex PCR
A. L. Szalanski,
C. E. Trammel,
A. D. Tripodi,
D. Cleary,
L. Rusert,
D. Downey
![](/ContentImages/journals/flen/99/4/4/WebImages/024.099.0400.cover.jpg)
Florida Entomologist
Vol. 99 • No. 4
December 2016
Vol. 99 • No. 4
December 2016
Apis mellifera
diagnóstico molecular
molecular diagnostics
parasite
parásito
tripanosoma
trypanosome