In this article, the efficacy of nitric oxide (NO) fumigation to control bulb mites in the genus Rhizoglyphus and its effects on germination and growth of flower bulbs were studied. Bulb mites on infested peanuts were fumigated with NO at different concentrations under ultralow oxygen conditions in 1.9-liter jars for 4–48 h at various temperatures ranging from 2 to 20 °C. Bulb mites were susceptible to NO fumigation treatment. Efficacy of fumigation treatment increased with increased concentration, treatment time, and temperature within the ranges tested. Complete control of bulb mites was achieved in 24-h fumigation with 3.0% and 5.0% NO at 10 °C and with 2.0% NO at 20 °C. A fumigation treatment with 3.0% NO at 20 °C for 24 h, which was stronger than necessary for bulb mite control, was tested on bulbs of four flower varieties to determine its safety on germination and growth of the flower bulbs. The NO fumigation treatments did not significantly affect germination and growth of flower bulbs. This study showed that NO fumigation was effective against bulb mites on peanuts and did not affect germination and growth of flower bulbs. Therefore, NO fumigation has potential for postharvest control of bulb mites on flower bulbs.