Registered users receive a variety of benefits including the ability to customize email alerts, create favorite journals list, and save searches.
Please note that a BioOne web account does not automatically grant access to full-text content. An institutional or society member subscription is required to view non-Open Access content.
Contact email@example.com with any questions.
New cell lines from embryos of Ephestia kuehniella were recently developed. Primary cultures were initiated in September 2002 from 2 to 4 day old eggs in either modified TC-100 or ExCell 400 medium. From these initial cultures, one, originally isolated in the Ex-Cell medium, produced sufficient cell growth to allow subcultivation and eventually led to the establishment of two cell strains, one that forms multicellular vesicles in suspension and one consisting of tightly attached epithelial-like cells. The strains were compared to an extract from E. kuehniella eggs by isozyme analysis and shown to be from the same species. Both strains were inoculated with various insect viruses, including nucleopolyhedroviruses from Autographa californica, Anagrapha falcifera, Anticarsa gemmatalis, Galleria mellonella, Heliothis armigera, Helicoverpa zea, Lymantria dispar, Plutella xylostella, and Rachoplusia ou. Both strains were highly susceptible to most of the nucleopolyhedroviruses (with the exception of the viruses from Helicoverpa zea and Lymantria dispar which did not show cytopathology to either cell strain) with large numbers of occlusion bodies produced in most of the inoculated cells. Our results suggest these new lines can be useful in biocontrol research.
nucleopolyhedrovirus, the prefix M or S refers to multiple or single nucleocapsids