The rediscovery of Calomyscus hotsoni in Pakistan is reported. This species had previously been known from a series of specimens taken at the type locality in southwestern Pakistan in 1918 and a series of specimens from 4 localities in southeastern Iran collected in 1962 and reported as this taxon in 2005. Specimens of C. hotsoni were collected from 4 additional locations in southern Pakistan between 1996 and 1999. Partial sequence data from cytochrome b support the recognition of C. hotsoni as a distinct species from the other Calomyscus found in Pakistan, C. baluchi. Material collected from near the type localities of C. hotsoni and C. baluchi demonstrate significant differences in 5 cranial measurements and all external measurements other than ear height. C. hotsoni can be differentiated from C. baluchi by its smaller hind foot. The known distribution of C. hotsoni is greatly expanded and some ecological observations about the species also are reported. Examination of data presented on the distribution and on variation in local abundance suggests that C. hotsoni is currently neither endangered nor threatened.