First Description of Polytene Chromosomes in Biting Midges (Diptera: Ceratopogonidae)

Aleksandra Urbanek; Ryszard Szadziewski; Wojciech Giłka; Art Borkent

doi:10.1603/ME12059

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Aleksandra Urbanek,^1,* Ryszard Szadziewski,¹ Wojciech Giłka,¹ Art Borkent²

¹1Department of Invertebrate Zoology, University of Gdańsk, Al. Pilsudskiego 46, 81-378 Gdynia, Polan
²3Research Associate of the Royal British Columbia Museum, American Museum of Natural History, and In

^*Corresponding author, e-mail: dokale@ug.edu.pl.

Abstract

Polytene chromosomes are described from secretory cells in larvae of Forcipomyia nigra (Winnertz). They are present in large glandular-trichogen cells at the bases of secretory setae and in midgut cells that were observed by transmission electron microscopy and light microscopy. Polytene chromosomes, isolated from the glandular-trichogen cells using aceto-orcein squash technique, measure 50–200 µm, have braid-like strands of chromatin and no bands, features that are unique within the Culicomorpha.

Citation Download Citation

Aleksandra Urbanek, Ryszard Szadziewski, Wojciech Giłka, and Art Borkent "First Description of Polytene Chromosomes in Biting Midges (Diptera: Ceratopogonidae)," Journal of Medical Entomology 50(2), 458-461, (1 March 2013). https://doi.org/10.1603/ME12059

Received: 14 March 2012; Accepted: 1 August 2012; Published: 1 March 2013