Polytene chromosomes are described from secretory cells in larvae of Forcipomyia nigra (Winnertz). They are present in large glandular-trichogen cells at the bases of secretory setae and in midgut cells that were observed by transmission electron microscopy and light microscopy. Polytene chromosomes, isolated from the glandular-trichogen cells using aceto-orcein squash technique, measure 50–200 µm, have braid-like strands of chromatin and no bands, features that are unique within the Culicomorpha.
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Vol. 50 • No. 2