Food safety is an important issue for the development of the national economy and society. Studying regional food supply and demand from the perspective of land resource carrying capacity can provide new references for regional resource sustainability. This study uses the data from farmer and herdsmen household questionnaires, statistical data, land use data, and other sources to construct a land resource carrying capacity (LCC) assessment framework, targeting the food supply and demand of residents in representative areas, specifically the typical grassland pastoral areas, sandy pastoral areas and agro-pastoral areas on the Xilin Gol grassland transects. The three food nutritional indicators of calories, protein and fat were selected for analyzing the balance of land resource carrying capacity. We found that: 1) Along the Xilin Gol grassland, the main local food supply showed a shift from meat and milk to grains, vegetables and fruits. 2) From north to south along the grassland transects, the calorie intake increased gradually, while the intake of protein and fat was highest in pastoral areas and lowest in agricultural areas. 3) The overall land resource carrying capacity of the Xilin Gol grassland transects was in a surplus state, but the land carrying capacity of typical grassland pastoral area was higher than the two other types of areas. This study provides an empirical reference for the sustainable development of regional food nutrition.