Traditional sources of carbon dioxide (CO₂), dry ice, and compressed gas, were tested against 3 combinations of food-grade reagents known to generate CO₂ using a compact, lightweight generator delivery system with Centers for Disease Control and Prevention light traps. Three 6 × 6 Latin square trials were completed near the Florida Gulf Coast in the Lower Suwannee Wildlife Refuge during the summer of 2013, collecting a total of 31,632 female mosquitoes. Treatments included dry ice, compressed CO₂ gas, a control trap (no CO₂), citric acid sodium bicarbonate, vinegar sodium bicarbonate, and yeast sugar. Decreasing order of trap collections (treatment mean number of mosquitoes per trap night ± standard error) were dry ice 773.5 (±110.1) > compressed gas 440.7 (±42.3) > citric acid sodium bicarbonate 197.6 (±30.4), yeast sugar 153.6 (±27.4) > vinegar sodium bicarbonate 109.6 (±16.2) > control 82.4 (±14.0). A 2-way Kruskal–Wallis analysis by treatment, site, and treatment × site interaction identified significant differences between all treatments. Although dry ice and compressed CO₂ gas collected significantly more mosquitoes than other combinations (P < 0.05), use of citric acid and sodium bicarbonate or yeast and sugar greatly outperformed unbaited traps and offer a good alternative to dry ice and compressed gas in areas where these agents are not readily available or are difficult to obtain due to logistical constraints. An inexpensive, portable CO₂ generator for use with food-grade reagents is described.