Dengue and other vector-borne diseases are of great public health importance in Colombia. Vector surveillance and control activities are often focused at the household level. Little is known about the importance of nonhousehold sites, including schools, in maintaining vector-borne disease transmission. The objectives of this paper were to determine the mosquito species composition in rural schools in 2 municipalities in Colombia and to assess the potential risk of vector-borne disease transmission in school settings. Entomological surveys were carried out in rural schools during the dry and rainy seasons of 2011. A total of 12 mosquito species were found: Aedes aegypti, Anopheles pseudopunctipennis, Culex coronator, Cx. quinquefasciatus, and Limatus durhamii in both immature and adult forms; Ae. fluviatilis, Cx. nigripalpus, Cx. corniger, and Psorophora ferox in immature forms only; and Ae. angustivittatus, Haemagogus equinus, and Trichoprosopon lampropus in adult forms only. The most common mosquito species was Cx. quinquefasciatus. Classrooms contained the greatest abundance of adult female Ae. aegypti and Cx. quinquefasciatus. The most common Ae. aegypti breeding sites were containers classified as “others” (e.g., cans), followed by containers used for water storage. A high level of Ae. aegypti infestation was found during the wet season. Our results suggest that rural schools are potentially important foci for the transmission of dengue and other mosquito-borne diseases. We propose that public health programs should be implemented in rural schools to prevent vector-borne diseases.

Monitoring adult mosquito populations provides information that is critical for assessing risk of vector-borne disease transmission. The recently developed Suna trap was found to be a very effective trap when baited with an attractive odor blend. A modification of this trap was tested to improve its function as a tool for monitoring mosquito populations, including Anopheles coluzzii (An. gambiae sensu stricto molecular form M), Aedes aegypti, and Culex pipiens. The modified Suna trap (Suna-M) was altered by changing the position of the catch bag and the inclusion of a holding chamber in attempts to increase trapping efficacy and enhance the survival of mosquitoes. Each adaptation was tested in a dual-choice setup in a climate-controlled room against the original Suna trap and against 4 standard monitoring methods: the BG-sentinel (BGS), Centers for Disease Control and Prevention (CDC) light trap, Mosquito Magnet X (MM-X) trap, and human landing catch (HLC). No differences in trapping efficacy were observed between the original Suna trap and modified version; however, a version in which the funnel was extended with a box and supplemented with moistened cotton wool increased mosquito survival from 6.5% to 78.0% over 24 h. The HLC and BGS trap outperformed the Suna-M trap, whereas the MM-X and commonly used CDC light trap performed significantly less well than the Suna-M trap in the dual-choice setup. The performance of the Suna-M trap equaled the performance of the original Suna trap and could therefore be used for monitoring purposes. Although the HLC and BGS trap achieved higher catch sizes, the Suna trap has the advantage that it is standardized, does not place humans at risk, and is weather resistant. Field studies should be conducted to confirm that the Suna-M trap, baited with the odor blend, is an efficient and standardized tool to measure both indoor and outdoor disease transmission risk for a range of vector-borne diseases.

La Crosse virus (LACV) is a mosquito-borne virus and a major cause of pediatric encephalitis in the USA. La Crosse virus emerged in Tennessee and other states in the Appalachian region in 1997. We investigated LACV infection rates and seasonal abundances of the native mosquito vector, Aedes triseriatus, and 2 recently introduced mosquito species, Ae. albopictus and Ae. japonicus, in an emerging disease focus in Tennessee. Mosquitoes were collected using multiple trapping methods specific for Aedes mosquitoes at recent human case sites. Mosquito pools were tested via reverse transcriptase–polymerase chain reaction (RT-PCR) of the S segment to detect multiple Bunyamwera and California serogroup viruses, including LACV, as well as real-time RT-PCR of the M segment. A total of 54 mosquito pools were positive, including wild-caught adult females and laboratory-reared adults, demonstrating transovarial transmission in all 3 species. Maximum likelihood estimates (per 1,000 mosquitoes) were 2.72 for Ae. triseriatus, 3.01 for Ae. albopictus, and 0.63 for Ae. japonicus. We conclude that Ae. triseriatus and Ae. albopictus are important LACV vectors and that Ae. japonicus also may be involved in virus maintenance and transmission.

The Aedes mosquito vectors of dengue virus (DENV) and chikungunya virus (CHIKV) are attracted to specific host cues that are not generated by traditional light traps. For this reason multiple companies have designed traps to specifically target those species. Recently the standard trap for DENV and CHIKV vectors, the BG-Sentinel (BGS) trap, has been remodeled to be more durable and better suited for use in harsh field conditions, common during military operations, and relabeled the BG-Sentinel 2 (BGS2). This new trap was evaluated against the standard Centers for Disease Control and Prevention (CDC) light trap, Zumba Trap, and BG-Mosquitito Trap to determine relative effectiveness in collecting adult Aedes aegypti and Ae. albopictus. Evaluations were conducted under semifield and field conditions in suburban areas in northeastern Florida from May to August 2014. The BGS2 trap collected more DENV and CHIKV vectors than the standard CDC light trap, Zumba Trap, and BG-Mosquitito Trap, but attracted fewer species, while the BG-Mosquitito Trap attracted the greatest number of mosquito species.

Traditional sources of carbon dioxide (CO₂), dry ice, and compressed gas, were tested against 3 combinations of food-grade reagents known to generate CO₂ using a compact, lightweight generator delivery system with Centers for Disease Control and Prevention light traps. Three 6 × 6 Latin square trials were completed near the Florida Gulf Coast in the Lower Suwannee Wildlife Refuge during the summer of 2013, collecting a total of 31,632 female mosquitoes. Treatments included dry ice, compressed CO₂ gas, a control trap (no CO₂), citric acid sodium bicarbonate, vinegar sodium bicarbonate, and yeast sugar. Decreasing order of trap collections (treatment mean number of mosquitoes per trap night ± standard error) were dry ice 773.5 (±110.1) > compressed gas 440.7 (±42.3) > citric acid sodium bicarbonate 197.6 (±30.4), yeast sugar 153.6 (±27.4) > vinegar sodium bicarbonate 109.6 (±16.2) > control 82.4 (±14.0). A 2-way Kruskal–Wallis analysis by treatment, site, and treatment × site interaction identified significant differences between all treatments. Although dry ice and compressed CO₂ gas collected significantly more mosquitoes than other combinations (P < 0.05), use of citric acid and sodium bicarbonate or yeast and sugar greatly outperformed unbaited traps and offer a good alternative to dry ice and compressed gas in areas where these agents are not readily available or are difficult to obtain due to logistical constraints. An inexpensive, portable CO₂ generator for use with food-grade reagents is described.

Unlike the application of Bacillus thuringiensis israelensis (Bti) for the control of larval mosquitoes, studies of its effects on adults, for its possible use in attractive toxic sugar baits, have resulted in conflicting results. Five species have shown a decrease in adult survival due to Bti ingestion, whereas adults of Anopheles arabiensis have not. We sought to determine if ingestion of Bti by adults of Anopheles gambiae, a sibling species of An. arabiensis, increases their mortality. Laboratory-reared adults were provided continuously from emergence with water only, a sucrose solution, or a Bti suspension in sucrose solution. After 3 days, the Bti suspension was replaced with untainted sucrose solution. The mosquitoes with only water were all dead by day 3. The survivorships of those in the sucrose and sucrose-Bti treatments were insignificantly different, both with an LT₅₀ (Lethal Time, time until 50% of individuals died) of 25 days. The results support the conclusion that adult survivorship of An. gambiae–complex members is unaffected by the ingestion of Bti in sugar meals.

The authors of a recently published paper summarized the development of a regression model for ground-based ultra-low volume applications, suggesting that their model was sufficiently verified that it could be used extensively for mosquito control. These authors claimed that their statistical model was superior in its predictive capability to the extensively developed and Environmental Protection Agency–validated AGDISP mechanistic model. In this technical review, the assumptions, reduction and interpretation of data, and conclusions reached with regard to their model are discussed, and explicit misstatements and incorrect mathematical relationships are pointed out. Two published versions of the model regression equation give substantially different results without explanation. Petri dish collection was used for very small droplets, with no mention of collection efficiency. Meteorological data were misused based on manufacturer’s specification of instrument accuracy. We strongly disagree with many of the model results and show that the model misrepresents the actual behavior of aerosol sprays applied in the manner tested.

Larvae of Culex (Melanoconion) pilosus were collected during February–April 2014 in temporary pools in “Bosques de Ezeiza,” a large forested park, near Buenos Aires city, Argentina. This is the first record in Buenos Aires Province, extending the distribution of this species 380 km to the south. Regarding habitat use, Cx. (Mel.) pilosus is a generalist, although a slight association of larval abundances with pools of lower pH and higher vegetation cover was observed. The comparison of larval instars of Cx. (Mel.) pilosus with those of other genera suggests a life-history strategy similar to that of floodwater mosquitoes.

We carried out dengue (DENV) and chikungunya virus (CHIKV) surveillance in wild populations of Aedes aegypti from Guerrero, Mexico, from 2012 to 2014 following a standard national protocol of the Mexican Dengue Control Program. A total of 284 pools (15–30 specimens/pool) of female mosquitoes were tested with real-time reverse transcriptase–polymerase chain reaction to detect DENV and CHIKV. We report for the 1st time the detection of CHIKV from field-collected mosquitoes at Acapulco and Juchitán in 2014. Results from DENV are also reported.

Filth fly control measures may be optimized with a better understanding of fly population dynamics measured throughout the day. We describe the modification of a commercial motorized sequential mosquito trap to accept liquid odorous bait and leverage a classic inverted-cone design to passively confine flies in 8 modified collection bottles corresponding to 8 intervals. Efficacy trials in a hot-arid desert environment indicate no significant difference (P  =  0.896) between the modified sequential trap and a Rid-Max® fly trap.

Because it is often logistically impossible to monitor all catch basins within an operational area, local mosquito control programs will preemptively treat catch basins with larvicides each season. However, these larvicides can, ostensibly, be considered water quality pollutants. To experimentally reduce the use of larvicides toward improving water quality, 30 basins within a small 0.7-km² residential area were monitored weekly for the presence of larvae and pupae for 14 wk in the summer of 2013. Once a basin was found to reach a threshold of 12 mosquitoes per dip sample, it received a FourStar® Briquet (a 180-day briquet formulation of 6% Bacillus sphaericus and 1% B. thuringiensis israelensis). Each week a FourStar-treated basin surpassed this threshold, it was treated with an application of CocoBear™ oil (10% mineral oil). By the end of trials, all but one basin received a briquet and 13 required at least 4 treatments of CocoBear, suggesting that preemptive treatment is appropriate for the study area.

The 25th Annual Latin American Symposium presented by the American Mosquito Control Association (AMCA) was held as part of the 81st Annual Meeting in New Orleans, LA, in March 2015. The principal objective, as for the previous 24 symposia, was to promote participation in the AMCA by vector control specialists, public health workers, and academicians from Latin America. This publication includes summaries of 24 presentations that were given orally in Spanish by participants from Colombia, Mexico, and the USA. Topics addressed in the symposium included: surveillance, operations, ecology, chemical control, studies of dengue viruses, and insecticide resistance. Insect vectors included Aedes, Culex, and Anopheles mosquitoes in addition to phlebotomine sand flies and triatomine bugs.