A chronological list of species based on date of publication is provided for confirmed mosquito species in West Virginia. Five additional newly found species in the state are documented with collection data. Two previously misidentified or mislabeled published records are corrected. The number of confirmed species occurring in the state is now 35, and many more species are likely present, but undetected. Significant populations of 4 widely distributed known vector species, Aedes albopictus, Ae. japonicus japonicus, Ae. triseriatus, and Culex pipiens, in West Virginia signal an urgent need for additional studies, surveillance, and increased mosquito control efforts to help prevent future native and invasive arbovirus infections and outbreaks in local residents.