Encapsulated larvae of Trichinella spiralis were found in wild-trapped, Microtus pennsylvanicus and Sigmodon hispidus. In addition, Peromyscus leucopus and Mus musculus again were found infected. These mammals were trapped from a farm site in Henrico County, Virginia, remote from known potential sources of trichinosis. The possible zoonotic relationship between wild rodent trichinosis and swine trichinosis is discussed.