Campylocentrum amistadense Bogarín, a new orchid species from La Amistad International Park in Puntarenas, Costa Rica, is described and illustrated. It is most similar to C. brenesii Schltr., but differs in having linear, thin, subcoriaceous leaves, and glabrous perianth parts and inflorescence. The flowers are parallel to the inflorescence axis, the spur is shorter than the blade of the lip, longitudinally 3-keeled, and the callus is made up of densely pubescent rows along the sides of the midlobe but glabrous in the center. Campylocentrum tenellum Todzia is similar in size, but it is easily distinguished from the new species by the lacerate leaf sheaths and the pubescent upper surface of sepals. An updated taxonomic key to the species of Campylocentrum Benth. in Costa Rica is provided.