Chikungunya fever is a viral disease transmitted to humans by Aedes aegypti (L.), and Aedes albopictus (Skuse). The disease is characterized by sudden onset of fever usually accompanied by joint pain. The diagnostic is made by RT-qPCR of the virus genome or serological tests to confirm the presence of IgM and IgG anti-Chikungunya antibodies. A descriptive study was done with serum samples of patients with clinical symptoms of Chikungunya fever referred to the Laboratorio Estatal de Salud Publica de Nuevo Leon, northeastern Mexico. From 101 samples, 41 were positive for Chikungunya virus. The median age of the patients was 31 years old, of which 19 were females and 22 males. Thirty-one of the positive cases had traveled history to others states of Mexico: Guerrero (11), Chiapas (seven), Veracruz (seven), Oaxaca (four), Quintana Roo (one), and Yucatan (one). The most frequent signs in the patients were 92.7% (38) headache, 85.4% (35) myalgia, 73.2% (30) exanthema, 70.7% (29) arthralgia, 68.3% (28) retro-ocular pain. Five serum samples were registered with co-infection with dengue and Chikungunya virus. RNA was extracted from the positive samples, and cDNA was synthesized. The E2 segment of the virus genome was amplified by PCR, cloned, and genotyped. Chikungunya virus genotypes identified in patients from northeastern Mexico differed from those previously reported from other regions of Mexico.