Pathogenicity and virulence of three strains of entomopathogenic fungi, Isaria javanica (CHE-CNRCB 307), Metarhizium anisopliae (CHE-CNRCB 224), and Beauveria bassiana (CHE-CNRCB 168), and a check of 1 ml of sterile 0.03% Tween® 80 were evaluated on adult spotted-wing drosophila, Drosophila suzukii (Matsumura). Lethal concentration 50 (LC₅₀) of entomopathogenic fungi was determined at five concentrations (1 × 10⁴, 1 × 10⁵, 1 × 10⁶, 1 × 10⁷, and 1 × 10⁸ conidia per ml^-1) and the lethal time (LT⁵⁰) at a dose of 1 × 10⁸ conidia per ml. The evaluated strains had different amounts of pathogenicity and virulence on adult D. suzukii. The I. javanica (CHE-CNRCB 307) strain had the largest significant differences with LC₅₀ of 1.6 × 10⁴ adult conidia per ml and LT₅₀ of 4,579 days. The M. anisopliae (CHE-CNRCB 224) and B. bassiana (CHE-CNRCB 168) strains were not significantly different compared to each other, with LC₅₀ of 3.1 × 10⁵ and 1.4 × 10⁶ adult conidia per ml and LT₅₀ of 18.16 and 15.3 days, respectively. Results provide information about entomopathogenic fungi and support for using it to control D. suzukii.