The two-spotted spider mite Tetranychus urticae Koch is a phytophagous spider mite that is now one of the most important pests of agricultural and economic crops worldwide. However, reports of T. urticae (green form) infection on cotton have been rare in China. Here we present two records of T. urticae (green form) on Gossypium hirsutum in China (Nanjing, Jiangsu Province and Shihezi, Xinjiang Uygur Autonomous Region). Because spider mites are difficult to identify, we confirmed the identification by both aedeagus morphology and PCR-RFLP (restriction fragment-length polymorphism) technology. These results also validate the usefulness of the RFLP technology for the identification of T. urticae.