Visualization of the infraciliature, which is an essential tool for the identification of ciliate species, has traditionally been obtained with silver proteinate methods. Since infraciliature is mainly composed of microtubules, we used the synthetic fluorescent taxoid FLUTAX as a method for ciliate identification. The main advantages of this method are the facility and rapidity of its application and the fact that no previous fixation and permeabilization processes are required. FLUTAX may also be used as a probe to follow morphogenetical changes in the microtubular cytoskeleton during the ciliate life cycle.
How to translate text using browser tools
1 July 2002
Direct Visualization of the Microtubular Cytoskeleton of Ciliated Protozoa with a Fluorescent Taxoid
LUCÍA ARREGUI,
CESAR MUÑOZ-FONTELA,
SUSANA SERRANO,
ISABEL BARASOAIN,
ALMUDENA GUINEA
ACCESS THE FULL ARTICLE
It is not available for individual sale.
This article is only available to subscribers.
It is not available for individual sale.
It is not available for individual sale.
The Journal of Eukaryotic Microbiology
Vol. 49 • No. 4
July 2002
Vol. 49 • No. 4
July 2002
Fluorescent Paclitaxel
FLUTAX
identification of ciliates
microtubules
staining methods
taxonomy