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1 July 2002 Direct Visualization of the Microtubular Cytoskeleton of Ciliated Protozoa with a Fluorescent Taxoid
LUCÍA ARREGUI, CESAR MUÑOZ-FONTELA, SUSANA SERRANO, ISABEL BARASOAIN, ALMUDENA GUINEA
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Abstract

Visualization of the infraciliature, which is an essential tool for the identification of ciliate species, has traditionally been obtained with silver proteinate methods. Since infraciliature is mainly composed of microtubules, we used the synthetic fluorescent taxoid FLUTAX as a method for ciliate identification. The main advantages of this method are the facility and rapidity of its application and the fact that no previous fixation and permeabilization processes are required. FLUTAX may also be used as a probe to follow morphogenetical changes in the microtubular cytoskeleton during the ciliate life cycle.

LUCÍA ARREGUI, CESAR MUÑOZ-FONTELA, SUSANA SERRANO, ISABEL BARASOAIN, and ALMUDENA GUINEA "Direct Visualization of the Microtubular Cytoskeleton of Ciliated Protozoa with a Fluorescent Taxoid," The Journal of Eukaryotic Microbiology 49(4), 312-318, (1 July 2002). https://doi.org/10.1111/j.1550-7408.2002.tb00376.x
Received: 27 August 2001; Accepted: 24 March 2002; Published: 1 July 2002
JOURNAL ARTICLE
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KEYWORDS
Fluorescent Paclitaxel
FLUTAX
identification of ciliates
microtubules
staining methods
taxonomy
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