The expression of mouse two-cell stage specific genes was studied using the modified DDRT-PCR method, which overcame the paucity of the experimental materials of preimplantation embryos. Embryo tissues equivalent to that of four blastomeres are sufficient for amplification of target genes as visualized using polyacrylamide gel. Sequence analyses and reverse Northern blots indicate that the genes of ATPase 6 and Ywhaz are expressed specifically in two-cell embryos. ATPase 6 is essential for one-cell to two-cell transition and plays an important role in establishment of oxidative phosphorylation, while Ywhaz is related to initiating cellular communication system.
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1 November 2003
Expression of Stage-Specific Genes during Zygotic Gene Activation in Preimplantation Mouse Embryos
Wenyong Li,
Jianke Zhang,
Weidong Yu,
Guisheng Liu,
Qingxuan Chen
DDRT-PCR
preimplantation embryo
reverse Northern blot
stage-specific gene expression