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1 June 2004 Synthesis and Release of Steroids in Intestines from Cynomolgus Monkeys (Macaca fascicularis)
Sachiko Miyamoto, Yang Chen, Shin'ichiro Nakamura, Tadashi Sankai, Takeo Machida, Takashi Yoshida
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Abstract

To examine the synthesis and release of steroids in intestinal tissues from cynomolgus monkeys (Macaca fascicularis), we performed the following experiments: 1) incubated prepared intestinal tissues with [3H]testosterone to study the conversion to other steroids; 2) used a radioimmunoassay to determine steroid levels in six segments of intestinal tissues and contents (duodenum, jejunum, ileum, cecum, colon, and rectum); 3) localized testosterone in the six intestinal segments by immunofluorescence histochemistry; and 4) determined steroid levels in feces from males and females of various ages by radio-immunoassay to examine a correlation between steroid levels and age or sex. In prepared intestinal tissues, testosterone was converted into androstenedione, 5α-dihydrotestosterone, and an unidentified substance; all of these steroids were detected in all segments of the intestinal tissues and contents by radioimmunoassay. Immunofluorescence showed that testosterone was located in all segments of intestinal epithelia. Androstenedione, testosterone, 5α-dihydrotestosterone, and the unidentified substance were also detected in feces, and their levels were not affected by the age or sex of the animal. The present findings in cynomolgus monkeys led us to conclude that 1) steroids were synthesized in the intestines; 2) intestinal steroids were released from the six intestinal tissues to the intestinal cavities and excreted outside the body with feces; and 3) intestinal steroids were released irrespective of age or sex of the animal. Intestinal steroids seem to be paracrine or exocrine agents and to have different characteristics from classical serum steroids.

Sachiko Miyamoto, Yang Chen, Shin'ichiro Nakamura, Tadashi Sankai, Takeo Machida, and Takashi Yoshida "Synthesis and Release of Steroids in Intestines from Cynomolgus Monkeys (Macaca fascicularis)," Zoological Science 21(6), 639-648, (1 June 2004). https://doi.org/10.2108/zsj.21.639
Received: 23 February 2004; Accepted: 1 April 2004; Published: 1 June 2004
KEYWORDS
5α-dihydrotestosterone
androstenedione
Cynomolgus monkey
Intestine
testosterone
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