A total of 667 specimens of pseudoscorpions, belonging to 15 species and four families were collected during 2014. Samples were taken from leaf litter at five localities in the Western Carpathians in the Slovak Karst National Park, Slovakia. The most abundant families were Neobisiidae (381 specimens) and Chthoniidae (275 specimens). Furthermore ten chernetid specimens and a single cheliferid deutonymph were recorded. Four of the 15 pseudoscorpion species were recorded in the Slovak Karst for the first time.