Investigations of invertebrates in nine hothouses (tropical gardens) in Switzerland provided some spiders as by-catch. In total, we collected 136 spiders, of which 65 specimens represent 14 species from six families (the rest of the collection consisted of unidentifiable juveniles). Nine species are alien for Europe, one originates from the Mediterranean and four species are native to Central Europe. Four species represent first records for the Swiss fauna: Nesticidae: Nesticella mogera (Yaginuma, 1972), Oonopidae: Diblemma donisthorpei O. , Ischnothyreus peltifer (Simon, 1892) and Prida sechellensis (). These four species are discussed briefly and documented with photos.