The mobility of human follicle mites may play an important role in the infestation and transmission of demodicidosis. In order to better understand the mobility of follicle mites and to test some miticides which may be responsible for the alteration of mobility of the mite, a Computer-Aided Microimage Processing and Analysis System (CAMPAS) for assaying the mobility of Demodex folliculorum was developed. The crawling velocity, as an index of mite mobility, showed a bimodal distribution with peaks from 0 to 40 µm/min and from 200 to 260 µm/min. Scarcely any mites with crawling velocity ranging from 80 to 160 µm/min were detected in these experiments. Adults of D. folliculorum showed two different mobility patterns: inactive and active. All mites with crawling velocity more than 200 µm/min were randomly exposed to (1) a mixture of 1% metronidazole plus 1% bezoar, (2) a mixture of 2% metronidazole plus 2% bezoar and (3) D-Hank's solution (as control). Based on the sequential data on the inhibitive effects of metronidazole-bezoar, it is suggested that both medicines are potent chemical inhibitors of the mobility of D. folliculorum.