The role of the electron transport chain, its associated proteins, and carrier molecules can be difficult for introductory biology students to understand. Role-playing activities provide a simple, active, cost-effective method for demonstrating and comprehending complex biological processes. This role-playing activity was designed to help introductory biology students learn the role of the electron transport chain in the synthesis of ATP. The activity can be completed within a single class period and, when combined with a post-activity writing assignment, can enhance student understanding of how the electron transport chain functions.